ارزیابی توان تجزیه لیگنوسلولز برخی از باکتری‌های جدا شده از انواع خاک و مواد در حال پوسیدگی

Recently, the use of microorganisms for conversion of plant biomass into many commercially valuable products has been studied. In this study, some soil samples, composting plant materials, and composted manure from different parts of Markazy province were collected to isolate and identify aerobic bacteria, which have been capable of utilizing lignin preparations as a sole source of carbon. Bacteria were isolated by using three types of lignin preparations and hot- water- extracted wheat stover and saw dusts. Two of the isolates, identified as Streptomyces sp. and Pseudomonades sp. were able to degrade wheat stover and saw dust lignin and polysaccharides. The growth rate of Streptomyces sp. and Pseudomonades sp. was higher in wheat stover fed cultures than the saw dust fed cultures. Results also showed that bacteria treatment of lignocelluloses and nitrogen supplementation of culture media had considerable effects on chemical composition of wheat stover and saw dust. All two bacteria genera increased crude protein, APPL, soluble lignin, and decreased carbohydrates and insoluble lignin of wheat stover and saw dust (P<0.01). Streptomyces showed more degradation ability than Pseudomonades, especially in culture media containing wheat stover. The utilization of yeast extract (as nitrogen source) improved degradation abilities of bacteria. The findings of this study indicate that these bacteria could be used for biological upgrading of agriculture residues in order to feed animals.